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anti cbs polyclonal antibody  (Proteintech)


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    Proteintech anti cbs polyclonal antibody
    Anti Cbs Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/14787+1+ap/CBS+Antibody/pmc13011990-50-57-63
    Average 96 stars, based on 161 article reviews
    anti cbs polyclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: L-cysteine mitigates busulfan-induced testicular injury through modulation of CBS/H 2 S axis
    Article Snippet: Anti-CBS , Proteintech , 14787-1-AP , 1:1000.

    Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2
    Article Snippet: Rabbit polyclonal anti-CBS antibody , Proteintech Group , 14787-1-AP (RRID: AB_2070970).

    Article Title: Comparative localization of cystathionine beta synthases and cystathionine gamma lyase in canine, non-human primate and human retina
    Article Snippet: CBS , Proteintech, 14787–1-AP , Rabbit , IgG Polyclonal , Human , 86% Dog 98% NHP , 1:1000 , 1:500.

    Western Blot:

    Article Title: Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia
    Article Snippet: H 2 S-generating enzyme markers , CSE , Mouse mAb , IF 1 : 50 WB 1 : 2000 , 60234-1-Ig , Proteintech, Rosemont, IL, USA. .. , CBS , Rabbit pAb , IF 1 : 50 WB 1 : 2000 , 14787-1-AP , Proteintech, Rosemont, IL, USA. .. 3-MST , Rabbit pAb , WB 1 : 500 , NBP1-82617 , Novus Biologicals, Centennial, CO, USA.

    Saline:

    Article Title: Cystine/Glutamate Xc - Antiporter Induction Compensates for Transsulfuration Pathway Repression by 2,3,7,8-Tetrachlorodibenzo- p -dioxin (TCDD) to Ensure Cysteine for Hepatic Glutathione Biosynthesis.
    Article Snippet: Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been associated with the induction of oxidative stress and the progression of steatosis to steatohepatitis with fibrosis.. It also disrupts metabolic pathways including one-carbon metabolism (OCM) and the transsulfuration pathway with possible consequences on glutathione (GSH) levels.. In this study, complementary RNAseq and metabolomics data were integrated to examine the hepatic transsulfuration pathway and glutathione biosynthesis in mice following treatment with TCDD every 4 days for 28 days.

    Incubation:

    Article Title: Cystine/Glutamate Xc - Antiporter Induction Compensates for Transsulfuration Pathway Repression by 2,3,7,8-Tetrachlorodibenzo- p -dioxin (TCDD) to Ensure Cysteine for Hepatic Glutathione Biosynthesis.
    Article Snippet: Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been associated with the induction of oxidative stress and the progression of steatosis to steatohepatitis with fibrosis.. It also disrupts metabolic pathways including one-carbon metabolism (OCM) and the transsulfuration pathway with possible consequences on glutathione (GSH) levels.. In this study, complementary RNAseq and metabolomics data were integrated to examine the hepatic transsulfuration pathway and glutathione biosynthesis in mice following treatment with TCDD every 4 days for 28 days.



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    anti cbs polyclonal antibody  (Proteintech)
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    Expression levels of <t>CBS,</t> <t>ALDH2,</t> AHCY, MAT1A and MTHFR proteins in liver tissues of rats in each group. (A) Expression levels of MAT1A protein in liver tissues of rats in each group (n = 3); (B) Expression levels of AHCY protein in liver tissues of rats in each group (n = 3); (C) Expression levels of MYHFR protein in liver tissues of rats in each group (n = 3); (D) Expression levels of ALDH2 protein in liver tissues of rats in each group (n = 3); (E) Expression levels of CBS protein in liver tissues of rats in each group (n = 3). Note: Data are expressed as the mean ± SD. “#” indicates that P < 0.05 compared with the normal group; “*” indicates that P < 0.05 compared with the model group.
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    antibodies targeting cbs  (Proteintech)
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    Expression levels of <t>CBS,</t> <t>ALDH2,</t> AHCY, MAT1A and MTHFR proteins in liver tissues of rats in each group. (A) Expression levels of MAT1A protein in liver tissues of rats in each group (n = 3); (B) Expression levels of AHCY protein in liver tissues of rats in each group (n = 3); (C) Expression levels of MYHFR protein in liver tissues of rats in each group (n = 3); (D) Expression levels of ALDH2 protein in liver tissues of rats in each group (n = 3); (E) Expression levels of CBS protein in liver tissues of rats in each group (n = 3). Note: Data are expressed as the mean ± SD. “#” indicates that P < 0.05 compared with the normal group; “*” indicates that P < 0.05 compared with the model group.
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    cbs  (Proteintech)
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    The protein expressions of H 2 S-producing enzymes – cystathionine <t>γ-lyase</t> <t>(CSE)</t> ( a ), cystathionine β-synthase <t>(CBS)</t> ( b ) – and their distribution (CBS – green, CSE - orange) ( c ) in the thoracic aorta in control lean (L, n=8), obese (O, n=8), and zofenopril-treated obese (OZ, n=8) rats. E - endothelium, M - tunica media. Blue fluorescence indicates cell nuclei stained with 4′,6′-diamidino-2-phenylindole (DAPI). Images captured at 20× magnification. The results are presented as mean ± S.E.M., and differences among groups were analyzed by one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 vs L; + p<0.5, ++ p<0.01 vs O.
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    rabbit polyclonal anti cbs antibody  (Proteintech)
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    The protein expressions of H 2 S-producing enzymes – cystathionine <t>γ-lyase</t> <t>(CSE)</t> ( a ), cystathionine β-synthase <t>(CBS)</t> ( b ) – and their distribution (CBS – green, CSE - orange) ( c ) in the thoracic aorta in control lean (L, n=8), obese (O, n=8), and zofenopril-treated obese (OZ, n=8) rats. E - endothelium, M - tunica media. Blue fluorescence indicates cell nuclei stained with 4′,6′-diamidino-2-phenylindole (DAPI). Images captured at 20× magnification. The results are presented as mean ± S.E.M., and differences among groups were analyzed by one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 vs L; + p<0.5, ++ p<0.01 vs O.
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    14787 1 ap  (Proteintech)
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    Proteintech 14787 1 ap
    The protein expressions of H 2 S-producing enzymes – cystathionine <t>γ-lyase</t> <t>(CSE)</t> ( a ), cystathionine β-synthase <t>(CBS)</t> ( b ) – and their distribution (CBS – green, CSE - orange) ( c ) in the thoracic aorta in control lean (L, n=8), obese (O, n=8), and zofenopril-treated obese (OZ, n=8) rats. E - endothelium, M - tunica media. Blue fluorescence indicates cell nuclei stained with 4′,6′-diamidino-2-phenylindole (DAPI). Images captured at 20× magnification. The results are presented as mean ± S.E.M., and differences among groups were analyzed by one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 vs L; + p<0.5, ++ p<0.01 vs O.
    14787 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression levels of CBS, ALDH2, AHCY, MAT1A and MTHFR proteins in liver tissues of rats in each group. (A) Expression levels of MAT1A protein in liver tissues of rats in each group (n = 3); (B) Expression levels of AHCY protein in liver tissues of rats in each group (n = 3); (C) Expression levels of MYHFR protein in liver tissues of rats in each group (n = 3); (D) Expression levels of ALDH2 protein in liver tissues of rats in each group (n = 3); (E) Expression levels of CBS protein in liver tissues of rats in each group (n = 3). Note: Data are expressed as the mean ± SD. “#” indicates that P < 0.05 compared with the normal group; “*” indicates that P < 0.05 compared with the model group.

    Journal: Frontiers in Pharmacology

    Article Title: Effect of gentiopicroside on endogenous formaldehyde homocysteine–pathway related proteins in rats with non-alcoholic steatohepatitis

    doi: 10.3389/fphar.2025.1700101

    Figure Lengend Snippet: Expression levels of CBS, ALDH2, AHCY, MAT1A and MTHFR proteins in liver tissues of rats in each group. (A) Expression levels of MAT1A protein in liver tissues of rats in each group (n = 3); (B) Expression levels of AHCY protein in liver tissues of rats in each group (n = 3); (C) Expression levels of MYHFR protein in liver tissues of rats in each group (n = 3); (D) Expression levels of ALDH2 protein in liver tissues of rats in each group (n = 3); (E) Expression levels of CBS protein in liver tissues of rats in each group (n = 3). Note: Data are expressed as the mean ± SD. “#” indicates that P < 0.05 compared with the normal group; “*” indicates that P < 0.05 compared with the model group.

    Article Snippet: ROS, GSH, MDA, SOD, SAH, SAM, GST, CAT, GSH, HCY were obtained from Jiangsu Enzyme Immunoassay Biotechnology Co., LTD. Their item numbers are MM-88564O1, MM-20251R1, MM-2037H1, MM-20387R1, MM-50456H1, MM-0248H1, MM-21254R1, MM-20447R1, MM-20251R1 and MM-50456H1. β-actin antibody (Proteintech, 20536-1-AP); ALDH2 antibody (Proteintech, 15310-1-AP); MAT1A antibody (Proteintech, 12395-1-AP); MTHFR antibody (Proteintech, 26591-1-AP); CBS antibody (Proteintech, 14787-1-AP); AHCY antibody (Proteintech, 10757-2-AP); PVDF membrane with a thickness of 0.45 μm (IPVH00010, Millipore); Universal total RNA extraction reagent (U7431, Suzhou Youyi Landi Biotechnology Co., LTD.) Taq SYBR® Green qPCR Premix (Universal) (EG0117M, Jiangsu Bishi Mei Biotechnology Co., LTD.) All-in-One First-Strand Synthesis MasterMix (with dsDNase) was obtained from Jiangsu Bestmate Biotechnology Co., LTD. (item number (EG15133S).

    Techniques: Expressing

    Expression levels of CBS, ALDH2, AHCY, MAT1A and MTHFRmRNA in liver tissues of rats in each group (A) Expression levels of ALDH2 mRNA in liver tissues of rats in each group (n = 3); (B) Expression levels of MTHFRmRNA in liver tissues of rats in each group (n = 3); (C) Expression levels of CBS mRNA in liver tissues of rats in each group (n = 3); (D) Expression levels of AHCY mRNA in liver tissues of rats in each group (n = 3); (E) Expression levels of MAT1A mRNA in liver tissues of rats in each group (n = 3). Note: “#” indicates that P < 0.05 compared with the normal group; “*” indicates that P < 0.05 compared with the model group.

    Journal: Frontiers in Pharmacology

    Article Title: Effect of gentiopicroside on endogenous formaldehyde homocysteine–pathway related proteins in rats with non-alcoholic steatohepatitis

    doi: 10.3389/fphar.2025.1700101

    Figure Lengend Snippet: Expression levels of CBS, ALDH2, AHCY, MAT1A and MTHFRmRNA in liver tissues of rats in each group (A) Expression levels of ALDH2 mRNA in liver tissues of rats in each group (n = 3); (B) Expression levels of MTHFRmRNA in liver tissues of rats in each group (n = 3); (C) Expression levels of CBS mRNA in liver tissues of rats in each group (n = 3); (D) Expression levels of AHCY mRNA in liver tissues of rats in each group (n = 3); (E) Expression levels of MAT1A mRNA in liver tissues of rats in each group (n = 3). Note: “#” indicates that P < 0.05 compared with the normal group; “*” indicates that P < 0.05 compared with the model group.

    Article Snippet: ROS, GSH, MDA, SOD, SAH, SAM, GST, CAT, GSH, HCY were obtained from Jiangsu Enzyme Immunoassay Biotechnology Co., LTD. Their item numbers are MM-88564O1, MM-20251R1, MM-2037H1, MM-20387R1, MM-50456H1, MM-0248H1, MM-21254R1, MM-20447R1, MM-20251R1 and MM-50456H1. β-actin antibody (Proteintech, 20536-1-AP); ALDH2 antibody (Proteintech, 15310-1-AP); MAT1A antibody (Proteintech, 12395-1-AP); MTHFR antibody (Proteintech, 26591-1-AP); CBS antibody (Proteintech, 14787-1-AP); AHCY antibody (Proteintech, 10757-2-AP); PVDF membrane with a thickness of 0.45 μm (IPVH00010, Millipore); Universal total RNA extraction reagent (U7431, Suzhou Youyi Landi Biotechnology Co., LTD.) Taq SYBR® Green qPCR Premix (Universal) (EG0117M, Jiangsu Bishi Mei Biotechnology Co., LTD.) All-in-One First-Strand Synthesis MasterMix (with dsDNase) was obtained from Jiangsu Bestmate Biotechnology Co., LTD. (item number (EG15133S).

    Techniques: Expressing

    The mechanism diagram of endogenous formaldehyde participating in one-carbon metabolism leading to homocysteine accumulation and causing NASH. Note: formaldehyde (Endogenous FA); Glutathione (GSH) Mitochondrial aldehyde dehydrogenase 2 (ALDH2); Formate; Tetrahydrofolate (THF); 5, 10-methylenetetrahydrofolate (5, 10-CH2-THF); Methylenetetrahydrofolate reductase (MTHFR); Homocysteine (HCY); S-adenosine homocysteine (SAH); Reactive oxygen species (ROS); Non-alcoholic steatohepatitis (NASH); Methionine Cystathionine -β -synthase (CBS); 5-methyltetrahydrofolate (5-CH3-THF); S-adenosylmethionine (SAM); Methionine adenosyltransferase 1A (MAT1A); S-adenosine homocysteine hydrolase (AHCY).

    Journal: Frontiers in Pharmacology

    Article Title: Effect of gentiopicroside on endogenous formaldehyde homocysteine–pathway related proteins in rats with non-alcoholic steatohepatitis

    doi: 10.3389/fphar.2025.1700101

    Figure Lengend Snippet: The mechanism diagram of endogenous formaldehyde participating in one-carbon metabolism leading to homocysteine accumulation and causing NASH. Note: formaldehyde (Endogenous FA); Glutathione (GSH) Mitochondrial aldehyde dehydrogenase 2 (ALDH2); Formate; Tetrahydrofolate (THF); 5, 10-methylenetetrahydrofolate (5, 10-CH2-THF); Methylenetetrahydrofolate reductase (MTHFR); Homocysteine (HCY); S-adenosine homocysteine (SAH); Reactive oxygen species (ROS); Non-alcoholic steatohepatitis (NASH); Methionine Cystathionine -β -synthase (CBS); 5-methyltetrahydrofolate (5-CH3-THF); S-adenosylmethionine (SAM); Methionine adenosyltransferase 1A (MAT1A); S-adenosine homocysteine hydrolase (AHCY).

    Article Snippet: ROS, GSH, MDA, SOD, SAH, SAM, GST, CAT, GSH, HCY were obtained from Jiangsu Enzyme Immunoassay Biotechnology Co., LTD. Their item numbers are MM-88564O1, MM-20251R1, MM-2037H1, MM-20387R1, MM-50456H1, MM-0248H1, MM-21254R1, MM-20447R1, MM-20251R1 and MM-50456H1. β-actin antibody (Proteintech, 20536-1-AP); ALDH2 antibody (Proteintech, 15310-1-AP); MAT1A antibody (Proteintech, 12395-1-AP); MTHFR antibody (Proteintech, 26591-1-AP); CBS antibody (Proteintech, 14787-1-AP); AHCY antibody (Proteintech, 10757-2-AP); PVDF membrane with a thickness of 0.45 μm (IPVH00010, Millipore); Universal total RNA extraction reagent (U7431, Suzhou Youyi Landi Biotechnology Co., LTD.) Taq SYBR® Green qPCR Premix (Universal) (EG0117M, Jiangsu Bishi Mei Biotechnology Co., LTD.) All-in-One First-Strand Synthesis MasterMix (with dsDNase) was obtained from Jiangsu Bestmate Biotechnology Co., LTD. (item number (EG15133S).

    Techniques:

    The protein expressions of H 2 S-producing enzymes – cystathionine γ-lyase (CSE) ( a ), cystathionine β-synthase (CBS) ( b ) – and their distribution (CBS – green, CSE - orange) ( c ) in the thoracic aorta in control lean (L, n=8), obese (O, n=8), and zofenopril-treated obese (OZ, n=8) rats. E - endothelium, M - tunica media. Blue fluorescence indicates cell nuclei stained with 4′,6′-diamidino-2-phenylindole (DAPI). Images captured at 20× magnification. The results are presented as mean ± S.E.M., and differences among groups were analyzed by one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 vs L; + p<0.5, ++ p<0.01 vs O.

    Journal: Physiological Research

    Article Title: Vasoactive Effects of Chronic Treatment with ACE Inhibitor Zofenopril in Zucker Obese Diabetic Rats: The Role of Nitroso and Sulfide Signalization

    doi: 10.33549/physiolres.935722

    Figure Lengend Snippet: The protein expressions of H 2 S-producing enzymes – cystathionine γ-lyase (CSE) ( a ), cystathionine β-synthase (CBS) ( b ) – and their distribution (CBS – green, CSE - orange) ( c ) in the thoracic aorta in control lean (L, n=8), obese (O, n=8), and zofenopril-treated obese (OZ, n=8) rats. E - endothelium, M - tunica media. Blue fluorescence indicates cell nuclei stained with 4′,6′-diamidino-2-phenylindole (DAPI). Images captured at 20× magnification. The results are presented as mean ± S.E.M., and differences among groups were analyzed by one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 vs L; + p<0.5, ++ p<0.01 vs O.

    Article Snippet: Primary antibodies against CSE and CBS (Proteintech, Manchester, UK; diluted 1:100 in 1% goat serum) were applied overnight at 4 °C.

    Techniques: Control, Fluorescence, Staining